diff --git a/examples/openthread/pytest_otbr.py b/examples/openthread/pytest_otbr.py
index 3f851b5364..bfa04f4231 100644
--- a/examples/openthread/pytest_otbr.py
+++ b/examples/openthread/pytest_otbr.py
@@ -84,26 +84,44 @@ def fixture_Init_interface() -> bool:
 default_br_ot_para = ocf.thread_parameter('leader', '', '12', '7766554433221100', True)
 default_br_wifi_para = ocf.wifi_parameter('OTCITE', 'otcitest888', 10)
 default_cli_ot_para = ocf.thread_parameter('router', '', '', '', False)
+ESPPORT1 = os.getenv('ESPPORT1')
+ESPPORT2 = os.getenv('ESPPORT2')
+ESPPORT3 = os.getenv('ESPPORT3')
+
+PORT_MAPPING = {
+    'ESPPORT1': 'esp32h2',
+    'ESPPORT2': 'esp32s3',
+    'ESPPORT3': 'esp32c6'
+}
 
 
 # Case 1: Thread network formation and attaching
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|cli|br', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32h2|esp32s3'),
-        ('rcp_spi|cli|br_spi', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32h2|esp32c6|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|cli|br',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32h2|esp32s3',
+            f'{ESPPORT3}|{ESPPORT1}|{ESPPORT2}',
+            id='c6-h2-s3'
+        ),
+        pytest.param(
+            'rcp_spi|cli|br_spi',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32h2|esp32c6|esp32s3',
+            f'{ESPPORT1}|{ESPPORT3}|{ESPPORT2}',
+            id='h2-c6-s3'
+        ),
     ],
     indirect=True,
 )
@@ -163,17 +181,21 @@ def formBasicWiFiThreadNetwork(br:IdfDut, cli:IdfDut) -> None:
 
 # Case 2: Bidirectional IPv6 connectivity
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|cli|br', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32h2|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|cli|br',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32h2|esp32s3',
+            f'{ESPPORT3}|{ESPPORT1}|{ESPPORT2}',
+            id='c6-h2-s3'
+        ),
     ],
     indirect=True,
 )
@@ -212,17 +234,21 @@ def test_Bidirectional_IPv6_connectivity(Init_interface:bool, dut: Tuple[IdfDut,
 
 # Case 3: Multicast forwarding from Wi-Fi to Thread network
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|cli|br', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32h2|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|cli|br',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32h2|esp32s3',
+            f'{ESPPORT3}|{ESPPORT1}|{ESPPORT2}',
+            id='c6-h2-s3'
+        ),
     ],
     indirect=True,
 )
@@ -262,17 +288,21 @@ def test_multicast_forwarding_A(Init_interface:bool, dut: Tuple[IdfDut, IdfDut,
 
 # Case 4: Multicast forwarding from Thread to Wi-Fi network
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|cli|br', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32h2|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|cli|br',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32h2|esp32s3',
+            f'{ESPPORT3}|{ESPPORT1}|{ESPPORT2}',
+            id='c6-h2-s3'
+        ),
     ],
     indirect=True,
 )
@@ -313,17 +343,21 @@ def test_multicast_forwarding_B(Init_interface:bool, dut: Tuple[IdfDut, IdfDut,
 
 # Case 5: discover dervice published by Thread device
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|cli|br', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32h2|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|cli|br',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32h2|esp32s3',
+            f'{ESPPORT3}|{ESPPORT1}|{ESPPORT2}',
+            id='c6-h2-s3'
+        ),
     ],
     indirect=True,
 )
@@ -369,17 +403,21 @@ def test_service_discovery_of_Thread_device(Init_interface:bool, Init_avahi:bool
 
 # Case 6: discover dervice published by Wi-Fi device
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|cli|br', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32h2|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|cli|br',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32h2|esp32s3',
+            f'{ESPPORT3}|{ESPPORT1}|{ESPPORT2}',
+            id='c6-h2-s3'
+        ),
     ],
     indirect=True,
 )
@@ -432,17 +470,21 @@ def test_service_discovery_of_WiFi_device(Init_interface:bool, Init_avahi:bool,
 
 # Case 7: ICMP communication via NAT64
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|cli|br', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32h2|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|cli|br',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32h2|esp32s3',
+            f'{ESPPORT3}|{ESPPORT1}|{ESPPORT2}',
+            id='c6-h2-s3'
+        ),
     ],
     indirect=True,
 )
@@ -467,17 +509,21 @@ def test_ICMP_NAT64(Init_interface:bool, dut: Tuple[IdfDut, IdfDut, IdfDut]) ->
 
 # Case 8: UDP communication via NAT64
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|cli|br', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32h2|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|cli|br',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32h2|esp32s3',
+            f'{ESPPORT3}|{ESPPORT1}|{ESPPORT2}',
+            id='c6-h2-s3'
+        ),
     ],
     indirect=True,
 )
@@ -520,17 +566,21 @@ def test_UDP_NAT64(Init_interface:bool, dut: Tuple[IdfDut, IdfDut, IdfDut]) -> N
 
 # Case 9: TCP communication via NAT64
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|cli|br', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32h2|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|cli|br',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32h2|esp32s3',
+            f'{ESPPORT3}|{ESPPORT1}|{ESPPORT2}',
+            id='c6-h2-s3'
+        ),
     ],
     indirect=True,
 )
@@ -582,15 +632,26 @@ def test_TCP_NAT64(Init_interface:bool, dut: Tuple[IdfDut, IdfDut, IdfDut]) -> N
 @pytest.mark.esp32c6
 @pytest.mark.openthread_sleep
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('cli|sleepy', 2,
-         f'{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_sleepy_device/light_sleep")}',
-         'esp32h2|esp32c6'),
-        ('cli|sleepy', 2,
-         f'{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_sleepy_device/light_sleep")}',
-         'esp32c6|esp32h2'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'cli|sleepy',
+            2,
+            f'{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_sleepy_device/light_sleep")}',
+            'esp32h2|esp32c6',
+            f'{ESPPORT1}|{ESPPORT3}',
+            id='h2-c6'
+        ),
+        pytest.param(
+            'cli|sleepy',
+            2,
+            f'{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_sleepy_device/light_sleep")}',
+            'esp32c6|esp32h2',
+            f'{ESPPORT3}|{ESPPORT1}',
+            id='c6-h2'
+        ),
     ],
     indirect=True,
 )
@@ -628,16 +689,20 @@ def test_ot_sleepy_device(dut: Tuple[IdfDut, IdfDut]) -> None:
 
 # Case 11: Basic startup Test of BR
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|br', 2,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|br',
+            2,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32s3',
+            f'{ESPPORT3}|{ESPPORT2}',
+            id='c6-s3'
+        ),
     ],
     indirect=True,
 )
@@ -666,17 +731,21 @@ def test_basic_startup(dut: Tuple[IdfDut, IdfDut]) -> None:
 
 # Case 12: Curl a website via DNS and NAT64
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_bbr
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|cli|br', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32h2|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|cli|br',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32h2|esp32s3',
+            f'{ESPPORT3}|{ESPPORT1}|{ESPPORT2}',
+            id='c6-h2-s3'
+        ),
     ],
     indirect=True,
 )
@@ -704,15 +773,20 @@ def test_NAT64_DNS(Init_interface:bool, dut: Tuple[IdfDut, IdfDut, IdfDut]) -> N
 
 # Case 13: Meshcop discovery of Border Router
 @pytest.mark.supported_targets
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|br', 2,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|br',
+            2,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32s3',
+            f'{ESPPORT3}|{ESPPORT2}',
+            id='c6-s3'
+        ),
     ],
     indirect=True,
 )
@@ -761,17 +835,21 @@ def test_br_meshcop(Init_interface:bool, Init_avahi:bool, dut: Tuple[IdfDut, Idf
 
 # Case 14: Curl a website over HTTPS via DNS and NAT64
 @pytest.mark.supported_targets
-@pytest.mark.esp32h2
-@pytest.mark.esp32c6
 @pytest.mark.openthread_bbr
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('rcp_uart|cli|br', 3,
-         f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
-         'esp32c6|esp32h2|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'rcp_uart|cli|br',
+            3,
+            f'{os.path.join(os.path.dirname(__file__), "ot_rcp")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_cli")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_br")}',
+            'esp32c6|esp32h2|esp32s3',
+            f'{ESPPORT3}|{ESPPORT1}|{ESPPORT2}',
+            id='c6-h2-s3'
+        ),
     ],
     indirect=True,
 )
@@ -797,15 +875,20 @@ def test_https_NAT64_DNS(Init_interface:bool, dut: Tuple[IdfDut, IdfDut, IdfDut]
 
 # Case 15: Thread network formation and attaching with TREL
 @pytest.mark.supported_targets
-@pytest.mark.esp32c6
 @pytest.mark.openthread_br
 @pytest.mark.flaky(reruns=1, reruns_delay=1)
 @pytest.mark.parametrize(
-    'config, count, app_path, target', [
-        ('trel|trel', 2,
-         f'{os.path.join(os.path.dirname(__file__), "ot_trel")}'
-         f'|{os.path.join(os.path.dirname(__file__), "ot_trel")}',
-         'esp32c6|esp32s3'),
+    'config, count, app_path, target, port',
+    [
+        pytest.param(
+            'trel|trel',
+            2,
+            f'{os.path.join(os.path.dirname(__file__), "ot_trel")}'
+            f'|{os.path.join(os.path.dirname(__file__), "ot_trel")}',
+            'esp32c6|esp32s3',
+            f'{ESPPORT3}|{ESPPORT2}',
+            id='c6-s3'
+        ),
     ],
     indirect=True,
 )