diff --git a/tests/conftest.py b/tests/conftest.py
index 89e5361486e..1822254ddd0 100644
--- a/tests/conftest.py
+++ b/tests/conftest.py
@@ -21,7 +21,7 @@ import threading
 from typing import TYPE_CHECKING, Any, cast
 from unittest.mock import AsyncMock, MagicMock, Mock, _patch, patch
 
-from aiohttp import client
+from aiohttp import client, web_app
 from aiohttp.resolver import AsyncResolver
 from aiohttp.test_utils import (
     BaseTestServer,
@@ -469,6 +469,9 @@ def reset_globals() -> Generator[None]:
     frame.async_setup(None)
     frame._REPORTED_INTEGRATIONS.clear()
 
+    # Reset the aiohttp cache
+    web_app._cached_build_middleware.cache_clear()
+
     # Reset patch_json
     if patch_json.mock_objects:
         obj = patch_json.mock_objects.pop()